Biolegend, 315303, FITC anti-human CD46 Antibody, 25tests

CD46 is a transmembrane protein also known as complement membrane cofactor protein, MCP, and measles virus receptor. It is widely expressed on leukocytes, platelets, epithelial cells and fibroblasts. Multiple isoforms of CD46 have been reported with molecular weights ranging from 45-75 kD; CD46 binds complement components C3b and C4b and has also been reported to play a role in T cell regulation. In addition to the complement components, CD46 has been shown to interact with moesin, c-Src, tetraspanin 4, c-Yes, and integrin β1. CD46 is also known to be a cellular receptor for human measles virus and human herpes virus-6. Defects in CD46 have been associated with hemolytic-uremic syndrome. The MEM-258 antibody has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HPB-ALL T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone MEM-258 recognizes an epitope on the SCR4 (membrane-proximal SCR) domain of CD46. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting (non-reducing).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.Kickler K, et al. 2012. J immunol. 188:5303. PubMedBach P, et al. 2013. Cancer Res. 73:865. PubMedWu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:1056. PubMed
Product Citations: Wohlgemuth L, et al. 2022. Cells. 11: . PubMed Bach P, et al. 2013. Cancer Res. 73:865. PubMed Davies L, et al. 2016. Stem Cells Trans Med. 5: 1485 - 1495. PubMed Kickler K, et al. 2012. J Immunol. 188:5303. PubMed Tritschler H, et al. 2021. Cells. 10:. PubMed Ammour Y, et al. 2020. Viruses. 12:. PubMed Wu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:1056. PubMed
RRID: AB_2291290 (BioLegend Cat. No. 315304)
Structure: Transmembrane receptor, contains 4 SUSHI domains, multiple isoforms exist ranging in molecular weight from 45-75 kD. The larger isoform 65-75 kD is not expressed on peripheral blood cells.
Distribution: Widely expressed in leukocytes, platelets, epithelial cells and fibroblasts
Function: Binds complement components C3B and C4B with cofactor activity for the I-dependent cleavage of these proteins. May also play a role in T cell regulation
Interaction: Moesin, c-Src, complement component 3, complement component 4B, tetraspanin 4, c-Yes, integrin β1
Ligand/Receptor: C3B, C4B, Measles virus, human herpesvirus-6
Modification: Phosphorylation (Y368)
Cell Type: Leukocytes, Platelets, Epithelial cells, Fibroblasts
Biology Area: Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Dorig RE, et al. 1993. Cell 75:295.2. Cui W, et al. 1993. J. Immunol. 151:4137.3. Kemper C, et al. 2003. Nature 421:388.4. Marie JC, et al. 2002. Nat. Immunol. 3:659.
Gene ID: 4179
UniProt: View information about CD46 on UniProt.org
Clone: MEM-258
Regulatory Status: RUO
Other Names: Complement membrane cofactor protein, measles virus receptor, MCP
Isotype: Mouse IgG1, κ