Biolegend, 315809, APC/Cyanine7 anti-human CD177 Antibody, 25tests

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human granulocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.
Application References(PubMed link indicates BioLegend citation): Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed von Vietinghoff S, et al. 2007. Blood 109:4487. (IF) Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)
Product Citations: Zhou M, et al. 2024. Mil Med Res. 20:11. PubMed Saha R, et al. 2021. Sci Rep. 11:18849. PubMed
RRID: AB_2750078 (BioLegend Cat. No. 315809) AB_2750079 (BioLegend Cat. No. 315810)
Structure: uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution: Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)
Function: Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification: Glycosylated
Cell Type: Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002) 2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301. 3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108. 4. Temerinac S, et al. 2000. Blood 95:2569.
Gene ID: 57126
UniProt: View information about CD177 on UniProt.org
Clone: MEM-166
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype: Mouse IgG1, κ