Biolegend, 316009, PerCP/Cyanine5.5 anti-human CD52 Antibody, 25tests

CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression. The HI186 antibody is useful for flow cytometry and immunohistochemistry.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human tonsil
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations: Li K, et al. 2023. Leukemia. 37:308. PubMed
RRID: AB_2650806 (BioLegend Cat. No. 316009) AB_2650807 (BioLegend Cat. No. 316010)
Structure: Unusually small glycopeptide containing N-linked carbohydrate moiety, GPI-linked membrane protein, approximate molecular weight 7 kD
Distribution: Male reproductive tract (epididymis, seminal vesicle), T cells, B cells, macrophage/monocyte, eosinophil, erythrocytes, malignant lymphocytes
Function: Thought to play a role in carrying and orienting carbohydrates. Target for complement-mediated lysis and antibody-dependent cellular cytotoxicity, used as a depletion target for chronic lymphocytic leukemia/lymphoma therapy and immunosuppression.
Cell Type: B cells, Eosinophils, Erythrocytes, Macrophages, Monocytes, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997) Xia MQ, et al. 1991. Eur. J. Immunol. 21:1677. Kirchhoff C, et al. 1993. Mol. Reprod. Dev. 34:8. Xia MQ, et al. 1993. Biochem. J. 293:633.
Gene ID: 1043
UniProt: View information about CD52 on UniProt.org
Clone: HI186
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Cambridge pathology antigen 1 (CAMPATH-1), epididymal secretory protein 1
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.