Biolegend, 316308, Biotin anti-human β2-microglobulin Antibody, 100μg

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8 + T cells.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human β2-microglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.
Application References(PubMed link indicates BioLegend citation): Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
Product Citations: Mendoza JL, et al. 2020. eLife. 9:00. PubMed Tischer DK et al. 2019. eLife. 8 pii: e42498. PubMed
RRID: AB_493689 (BioLegend Cat. No. 316308)
Structure: Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Distribution: Leukocytes, platelets, endothelial cells, epithelial cells
Function: Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
Ligand/Receptor: CD3/TCR complex, CD8
Cell Type: Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13. 2. Williams DB, et al. 1989. J. Immunol. 142:2796. 3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533. 4. Williams A, et al. 2002. Tissue Antigens 59:3.
Gene ID: 567
UniProt: View information about beta2-microglobulin on UniProt.org
Clone: 2M2
Regulatory Status: RUO
Other Names: β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.