Biolegend, 316812, PE anti-human CD158b/j (KIR2DL2/L3/S2) Antibody, 100tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158b1, CD158b2, and CD158j are 33-38 kD two extracellular Ig domain glycoprotein that displays a long cytoplasmic tail with ITIM motifs for KIR2DL2/KIR2DL3 or a short cytoplasmic tail with an ITAM motif for KIR2DS2. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL2/KIR2DS2/KIR2DL3 has been reported to bind to certain HLA-C antigens on a target cell (HLA-C*01, HLA-C*03, HLA-C*07, for example).
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human KIR2DS2 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S22038E is able to block the binding of clone DX27 on target cells and is partially blocked by DX27. Based on in-house testing on different KIR-expressing transfectants, clone S22038E does not interact with other KIR protein family members.
RRID: AB_3720607 (BioLegend Cat. No. 316811) AB_3720607 (BioLegend Cat. No. 316812)
Structure: 33-38 kD two Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, subset of T cells
Function: NK cell regulation
Ligand/Receptor: Some HLA-C molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Vilches C, et al. 2002. Annu Rev Immunol. 92:107361. Middleton D, et al. 2010. Immunol. 123:8. Moesta AK, et al. 2012. Frontiers Immu. 3:336. David G, et al. 2013. Journal of Immu. 191:9.
Gene ID: 38033804100132285
UniProt: View information about CD158b CD158j on UniProt.org
Clone: S22038E
Regulatory Status: RUO
Other Names: CD158b1 (KIR2DL2, p58.2, NKAT6), CD158b2 (KIR2DL3, p58.3, NKAT2), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.