Biolegend, 316902, Purified anti-human CD268 (BAFF-R) Antibody, 100μg

B cell-activating factor receptor (BAFF-R) is a 19 kD type III membrane protein. It belongs to TNFR superfamily, also known as TNFRSF member 13C (TNFRSF13C), BAFF receptor 3 (BR3), or CD268. BAFF-R is expressed on mature B cells, B cell lymphoma, and T cell subset. BAFF-R is the major receptor for BAFF/BLys (or TALL-1, THANK) which binds to TACI and BCMA as well. The interaction of BAFF with BAFF-R promotes NF-κB activation and plays predominant roles in B-cell maturation and survival as well as costimulates T cell activation and proliferation. TRAF3 is a BAFF-R intracellularly associated protein, which negatively regulates BAFF-R-mediated NF-κB activation.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BAFF-R-L1.2 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Ng LG, et al.2004. J. Immunol. 173:807. (FC IHC) Personal communication. (Block)
RRID: AB_528985 (BioLegend Cat. No. 316902)
Structure: Type III transmembrane protein, TNF receptor superfamily, 19 kD
Distribution: Mature B cells, B cell lymphoma, T cell subset
Function: Induce B cell proliferation and immunoglobulin secretion, costimulate T cell activation
Ligand/Receptor: BAFF/BLys, associate with TRAF3
Cell Type: B cells, Leukemia, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Thompson JS, et al. 2001. Science 293:2108. 2. Ng LG, et al.2004. J. Immunol. 173:807. 3. Rodig SJ, et al. 2005. Human Pathol. 36:1113. 4. Ye Q, et al. 2004. Eur. J. Immunol. 34:2750. 5. Kayagaki N, et al. 2002 Immunity 17:515.
Gene ID: 115650
UniProt: View information about CD268 on UniProt.org
Clone: 11C1
Regulatory Status: RUO
Other Names: TNFRSF13C, BAFF-R, BAFFR, BR3, BAFF Receptor
Isotype: Mouse IgG1, κ