Biolegend, 317367, Cell-Vive™ GMP Ultra-LEAF™ Purified anti-HU CD3 RecAb SF, 100μg

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include
100μg
Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: 0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: GMP Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant Serum Free antibody purified by affinity chromatography
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Activ - Verified in house Costim - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. OKT3.Rec antibody can be used at 1 µg/mL of antibody in PBS alone or in combination with 1 µg/mL of anti-human CD28 antibody for T cell activation.
Application Notes: The OKT3.Rec recombinant antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. Clone OKT3.Rec can block the binding of clones SK7 and UCHT1. The OKT3.Rec antibody is able to induce T cell activation. This GMP Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.1 µm filtered) is recommended for highly sensitive assays.
Disclaimer: BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Antigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur J Immunol. 11:329. Lanier L, et al. 1986. J Immunol. 137:2501.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: OKT3.Rec
Other Names: T3, CD3ε
Isotype: Mouse IgG2a, κ