Biolegend, 317615, PE anti-human CD158b1/j (KIR2DL2/KIR2DS2) Antibody, 25tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158b1 and CD158j, also known as KIR2DL2 and KIR2DS2, respectively, are glycoproteins with two extracellular Ig domains. KIR2DL2 displays a long cytoplasmic tail with ITIM motifs and is 38 kD. KIR2DS2, with a short cytoplasmic tail containing an ITAM motif, is 33 kD in size.  Both KIR2DL2 and KIR2DS2 are expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL2 and KIR2DS2 have been reported to bind to certain HLA-C antigens on a target cell (HLA-C*01, HLA-C*03, HLA-C*07, for example).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human KIR2DL2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S22032A does not block the binding of clone DX27 on target cells and is not blocked by DX27.Based on in-house testing on different KIR-expressing transfectants, clone S22032A does not interact with other KIR protein family members.
RRID: AB_3720517 (BioLegend Cat. No. 317615) AB_3720517 (BioLegend Cat. No. 317616)
Structure: KIR2DL2 is 38 kD and has two extracellular Ig-like domains. KIR2DS2 is 33 kD and has two extracellular Ig-like domains.
Distribution: Subset of NK cells, subset of T cells
Function: NK cell regulation
Ligand/Receptor: Some HLA-C molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Vilches C, et al. 2002. Annu Rev Immunol. 92.107361. Middleton D, et al. 2010. Immunol. 123:8. Wisniewski A, et al. 2012. Human Immu. 73.9. Lin Z, et al. 2016. Cell Reports. 17.9.
Gene ID: 3803100132285
UniProt: View information about CD158b1/j on UniProt.org
Clone: S22032A
Regulatory Status: RUO
Other Names: CD158b1 (KIR2DL2, p58.2), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.