Biolegend, 318309, APC anti-human CD56 (NCAM) Antibody, 25tests

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Chen L, et al. 2023. Immunology. 169:204. PubMed Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed Wang T, et al. 2022. Front Immunol. 13:954121. PubMed Kenney DJ, et al. 2022. Cell Rep. 39:110714. PubMed Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed Siebert N, et al. 2014. PLoS One. 9:107692. PubMed Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed Junker F, et al. 2021. Cytometry A. 99:832. PubMed Davis Z, et al. 2011. J Vis Exp. 49: 2668. PubMed Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed Shokri MR, et al. 2019. Sci Rep. 9:10007. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed Laing AG, et al. 2020. Nat Med. 26:1623. PubMed Snyder J, et al. 2014. PLoS One. 9:107257. PubMed Miguel Reina‐Campos et al. 2019. Cancer cell. 35(3):385-400 . PubMed Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed Robin J, et al. 2015. J Vis Exp. 95: 52307. PubMed Huang Y, et al. 2020. FASEB J. 34:1768. PubMed Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed Pakula A, et al. 2018. Methods Mol Biol. 1889:01:00. PubMed Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed Ames E, et al. 2015. J Immunol. 195: 4010 - 4019. PubMed Rider P, et al. 2015. J Immunol. 195: 1705-1712. PubMed Smith SA, et al. 2019. Front Immunol. 9:3163. PubMed Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed Pereira PMR, et al. 2022. Nat Commun. 13:2526. PubMed Andersen J, et al. 2020. Cell. 183:1913. PubMed Al Tanoury Z, et al. 2020. Development. 147:00:00. PubMed Rosental B, et al. 2011. J Immunol. 187:5693. PubMed Su S et al. 2018. Cell. 175(2):442-457 . PubMed Vabret N, et al. 2022. iScience. 25:104599. PubMed Ma Y, et al. 2020. Cell Prolif. 53:e12802. PubMed Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed Yan W, et al. 2020. Biology of Reproduction. . PubMed Schlecker E, et al. 2014. Cancer Res. 74:3429. PubMed Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed Taouk G, et al. 2019. Sci Rep. 9:8756. PubMed Correia DV, et al. 2011. Blood. 118:992. PubMed Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed Al Tanoury Z, et al. 2020. Development. . PubMed Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed
RRID: AB_604098 (BioLegend Cat. No. 318309) AB_604106 (BioLegend Cat. No. 318310)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ