Biolegend, 318327, Brilliant Violet 421™ anti-human CD56 (NCAM) Antibody, 25tests

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Jo DH, et al. 2023. Mol Ther Methods Clin Dev. 29:173. PubMed Gunesch JT, et al. 2020. Elife. 9: . PubMed Gang A, et al. 2014. Blood Cancer J. 4:197. PubMed Boer M, et al. 2015. Clin Vaccine Immunol. 22: 778 - 788. PubMed Brito-Pérez Y, et al. 2021. Immun Inflamm Dis. 9:1541. PubMed de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed Huang A, et al. 2019. JCI Insight. 4. PubMed Offersen R, et al. 2016. J Virol. 90: 4441 - 4453. PubMed Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed Walk J, et al. 2019. Nat Commun. 10:874. PubMed Lee J, et al. 2020. Sci Rep. 10:17753. PubMed Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed Sharkey A, et al. 2015. J Immunol. 195: 3026 - 3032. PubMed Freeman A, et al. 2014. PLoS One. 9:110928. PubMed VanSaun M, et al. 2013. PLoS One. 8:73054. PubMed Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed Linedale R, et al. 2017. PLoS One. 12:e0175755. PubMed Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed Racle J et al. 2017. eLife. 6 pii: e26476. PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed Matsuda M, et al. 2020. Nature. 124:580. PubMed Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed Christensen SR, et al. 2019. J Virol. 93:8. PubMed Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed Shiba T, et al. 2019. J Allergy Clin Immunol. 144:1438. PubMed Lubaki N, et al. 2016. PLoS Pathog. 12:e1006031. PubMed
RRID: AB_10900228 (BioLegend Cat. No. 318327) AB_11218798 (BioLegend Cat. No. 318328)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.