Biolegend, 319001, Anti-human CD3 PE-Cyn5/CD4 PE/CD8 FITC Cocktail, 50tests

CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes. CD4: RPA-T4 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD8: RPA-T8 antibody reacts with CD8a, a 32-34 kD type I glycoprotein. CD8a forms a heterodimer or homodimer with CD8b. CD8 also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (express almost exclusively CD8a homodimers).
50tests
Verified Reactivity: Human
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: This reagent is a combination of PE/Cyanine5 conjugated UCHT1, PE conjugated RPA-T4 and FITC conjugated RPA-T8 at optimal concentration for three-color flow cytometric analysis.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.
Application: FC - Quality tested
Recommended Usage: For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.
Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Note: Single color controls are required to perform instrument compensation and are not included in the kit.
Product Citations: Greenberg J, et al. 2022. JCI Insight. Online ahead of print. PubMed
RRID: AB_316003 (BioLegend Cat. No. 319001)
Distribution: T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. 4. Center D, et al. 1996. Immunol. Today 17:476. 5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920916925
Clone: UCHT1; RPA-T4; RPA-T8;
Regulatory Status: RUO
Isotype: Mouse IgG1 (all clones)