Biolegend, 320918, PE/Cyanine7 anti-human MICA/MICB Antibody, 100tests

6D4 antibody reacts with a common epitope of the human nonclassical MHC class I chain-related protein A (MICA) and B (MICB), also known as PERB11.1 and PERB11.2. The MIC gene is located in MHC class I region. MICA/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate β2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN-α-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8 + α/β T cells and γ/δ T cells, as well as suppression of T cell proliferation. MICA/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases. The 6D4 antibody is able to block MIC mediated cytotoxicity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) applications include: immunohistochemistry2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation7, and blocking2,3 of MIC mediated cytotoxicity. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 320919 & 320120).
Application References(PubMed link indicates BioLegend citation): Groh V, et al. 1999. Science 279:1737. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. Groh V, et al. 2001. Nature Immunol. 2:255. Li Z, et al. 2000. Immunogenetics 51:246. Park EJ, et al. 2003. J. Immunol. 171:4131. Jinushi M, et al. 2003. J. Immunol. 171:5423. Wu J, et al. 2003. J. Immunol. 170:4196.
Product Citations: Rahim MMA, et al. 2020. J Virol. 94:00:00. PubMed
RRID: AB_2715910 (BioLegend Cat. No. 320917) AB_2715911 (BioLegend Cat. No. 320918)
Structure: 65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ß2-microglobulin, highly polymorphic
Distribution: Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells
Function: Activation of NK cells, subset of CD8+ α/ß T cells and γ/δ T cells, suppression of T cell proliferation
Ligand/Receptor: NKG2D
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445. 2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. 3. Jinushi M, et al. 2003. J. Immunol. 170:1249. 4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805. 5. Groh V, et al. 1999. Science 279:1737.
Gene ID: 1005074364277
UniProt: View information about MICA MICB on UniProt.org
Clone: 6D4
Regulatory Status: RUO
Other Names: PERB11, MIC, MHC class I chain-related protein A, MICA, PERB11.1, MHC class I chain-related protein B, MICB, PERB11.2
Isotype: Mouse IgG2a, κ