Biolegend, 322605, PE anti-human CD62E Antibody, 25tests

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Choi YW, et al. 2009. Vascul Pharmacol. 51:215. PubMed
Product Citations: Choi Y, et al. 2009. Vascul Pharmacol. 51:215. PubMed Subramaniam S, et al. 2021. Blood Adv. 5:2760. PubMed Dayang EZ, et al. 2021. Biomed Pharmacother. 133:111073. PubMed Dayang EZ, et al. 2019. Front Immunol. 10:1169. PubMed Kumboyono K, et al. 2021. Vasc Health Risk Manag. 17:103. PubMed Vanoni G, et al. 2021. eLife. 10:00. PubMed Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
RRID: AB_536007 (BioLegend Cat. No. 322605) AB_536008 (BioLegend Cat. No. 322606)
Structure: Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution: Highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression); can also be expressed on endothelial cells in the skin, bone marrow and placenta.
Function: Involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor: Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported in interact with CD62E
Cell Type: Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Collins T, et al. 1991. J. Biol. Chem. 266:2466. 2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238. 3. Berg EL, et al. 1991. J. Exp. Med. 174:1461. 4. Lawrence MB, et al. 1993. J. Immunol. 151:6338. 5. Walz G, et al. 1990. Science 250:1132.
Gene ID: 6401
UniProt: View information about CD62E on UniProt.org
Clone: HCD62E
Regulatory Status: RUO
Other Names: E-selectin, ELAM-1, LECAM-1
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.