Biolegend, 323219, Brilliant Violet 421™ anti-human CD105 Antibody, 25tests

CD105 is also known as Endoglin. It is a type I integral membrane homodimer protein with subunits of 90 kD found on vascular endothelial cells and syncytiotrophoblasts of placenta. CD105 is weakly expressed on stromal fibroblasts. It is also expressed on activated monocytes and tissue macrophages. Expression of CD105 is increased on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation. CD105 is a component of the TGF-β receptor system in human umbilical vein endothelial cells and binds TGF-β1 and β3 with high affinity but does not bind to TGF-β2.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: L-cells transfected with human CD105
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Bühring HJ, et al. 1991. Leukemia 5:841. Vogel W, et al. 2002. Haematologica 88:126. Osaka M, et al. 2011. Brain Res. 9:1343. PubMed HonMou O, et al. 2011. Brain. 134:1790. PubMed Herrera MB, et al. 2013. Hepatology. 57:311. PubMed Iohara K, et al. 2014. Exp Gerontol. 52:39. PubMed
Product Citations: Li Y, et al. 2020. Front Cell Dev Biol. 8:604021. PubMed
RRID: AB_2562242 (BioLegend Cat. No. 323219)
Structure: Associates with TGF-ß receptor I and II, homodimeric type I integral membrane protein, 90 kD
Distribution: Endothelial cells, activated monocytes/macrophages, bone marrow stromal cells, hematopoietic stem/progenitor cells
Function: Modulates cellular response to TGF-ß, involved in vascular development and remodelling
Ligand/Receptor: TGF-ß1, TGF-ß3
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area: Angiogenesis, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York. 2. Pierelli L, et al. 2001. Leuk. Lymphoma 42:1195.
Gene ID: 2022
UniProt: View information about CD105 on UniProt.org
Clone: 43A3
Regulatory Status: RUO
Other Names: Endoglin
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.