Biolegend, 323506, PE anti-human CD140a (PDGFRα) Antibody, 100tests

The 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, and PDGFRα. CD140a is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140a contains three immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight of approximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cells and has been implicated in the development of some tumors including basal cell carcinoma and gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well as protease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has been shown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others as integrin β3, caveolin-1, and nexin sorting molecules. The PDGFRα is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 16A1 antibody has been shown to be useful for flow cytometric detection of CD140a.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH 3T3 cells transfected with human PDGFRalpha
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Miyazaki S et al. In:Leukocyte Typing VI Kishimoto et al. Eds, Garland Publishing Inc, New York 1998 pp 3-20. Lottaz C, et al. 2010. Cancer Res. 70:2030. PubMed Ricono JM, et al. 2009. Am. J. Physiol. Renal Physiol. 296:F406. (IF) Guarnerio J, et al. 2015. Cancer Discov. 5:396. PubMed
Product Citations: Li W, et al. 2023. iScience. 26:106156. PubMed Tamaoki N, et al. 2023. Cell Rep Methods. 3:100460. PubMed De Bacco F, et al. 2021. Cell Reports. 36(4):109455. PubMed Fujimoto T, et al. 2020. Cell Rep. 32:108130. PubMed Baio JM, et al. 2017. Transplant Direct. 3:e153. PubMed Yamanaka S, et al. 2021. STAR Protocols. 2(1):100314. PubMed Saison‐Ridinger M, et al. 2017. PLoS One. 10.1371/journal.pone.0189051. PubMed Liau BB et al. 2017. Cell stem cell. 20(2):233-246 . PubMed Koide M, et al. 2018. Tohoku J Exp Med. 244:15:00. PubMed Lottaz C, et al. 2010. Cancer Res. 70:2030. PubMed Guarnerio J, et al. 2015. Cancer Discovery. 5:396. PubMed Xu S, et al. 2018. Mol Cancer Ther. 17:1291. PubMed Liu X, et al. 2022. Cell Discov. 8:58. PubMed Dmitrieva RI, et al. 2019. Stem Cells Int. 2019:5690345. PubMed Morales Pantoja IE, et al. 2020. PLoS One. 15:e0233980. PubMed Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed Zeltner N, et al. 2016. Nat Med. 22:1421-1427. PubMed
RRID: AB_2252226 (BioLegend Cat. No. 323505) AB_2268113 (BioLegend Cat. No. 323506)
Structure: Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family.
Distribution: Widely expressed on a variety of mesenchymal-derived cells.
Function: Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Knock-out studies have implicated an essential role for CD140a in kidney development. Has been implicated in basal cell carcinoma and gastric stromal cell tumors.
Interaction: Interacts with Crk, as well as a variety of adaptor molecules and signaling intermediates (Grb2, Grb14, SHP2, others). Has also been shown to associate with integrin β3, caveolin-1, and nexin sorting molecules
Ligand/Receptor: Binds to A-chain containing PDGF molecules and protease-activated PDGF-C molecules
Modification: Multiple tyrosine phosphorylation sites (Y720, Y731, Y742, Y754, Y762, Y767, Y768, Y988, Y993, Y1018)
Cell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435. 2. Gilbertson DG, et al. 2001. J. Biol. Chem. 276:27406. 3. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771. 4. Rupp E, et al. 1994. Eur. J. Biochem. 225:29.
Gene ID: 5156
UniProt: View information about CD140a on UniProt.org
Clone: 16A1
Regulatory Status: RUO
Other Names: Platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, PDGFRα, PDGFRa, PDGF receptor alpha
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.