Biolegend, 323902, Purified anti-human CD172b (SIRPβ) Antibody, 100μg

The B4B6 antibody recognizes human CD172b also known as signal-regulatory protein beta, and SIRP-beta. CD172b is a member of the signal regulatory protein family and immunoglobulin superfamily. CD172b contains a single pass transmembrane region with immunoglobulin-like domains and unlike CD172a, this receptor lacks a cytoplasmic SHP-2 binding domain. CD172b is a disulfide-linked homodimer with a predicted molecular weight of approximately 43 kD. Two isoforms of CD172b are produced by alternative splicing. CD172b is expressed on myeloid cells including monocytes and dendritic cells where it functions in the negative regulation of tyrosine-kinase coupled signaling processes. CD172b has been shown to regulate neutrophil transepithelial migration. CD172b interacts with DAP12 and Syk kinase (through TYROBP). The B4B6 antibody has been shown to be useful for flow cytometry.
100μg
Verified Reactivity: Human
Reported Reactivity: Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH-3T3 cells transfected with SIRPbeta
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Seiffert M, et al. 2001. Blood 97:2741.
Product Citations: Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID: AB_2301799 (BioLegend Cat. No. 323902)
Structure: Member of the signal regulatory protein family and immunoglobulin superfamily. Single pass transmembrane receptor with immunoglobulin-like domains; lacks cytoplasmic SHP-2 binding domain. Disulfide-linked homodimer, predicted MW ~43 kD.
Distribution: Myeloid cells, monocytes, dendritic cells
Function: Negative regulation of tyrosine-kinase coupled signaling processes. Regulates neutrophil transepithelial migration
Interaction: DAP12 and Syk (through TYROBP)
Cell Type: Dendritic cells, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kharitonenkov A, et al. 1997. Nature 386:181. 2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147.
Gene ID: 10326
UniProt: View information about CD172b on UniProt.org
Clone: B4B6
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: SIRPb, CD172 antigen-like family member B
Isotype: Mouse IgG1, κ