Biolegend, 324708, APC anti-human erbB3/HER-3 Antibody, 100tests

The 1B4C3 monclonal antibody recognizes human erbB3/HER3 also know as oncogene erbB3, tyrosine kinase cell surface receptor HER3, and C-erbB3. erbB3/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight approximately 148 kD. Signaling through the erbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erb3/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to be useful for flow cytometric detection of human erbB3/HER3.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH-3T3 cells transfected with human erbB3/HER3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Guo D, et al. 2022. Bio Protoc. 12:. PubMed De Bacco F, et al. 2021. Cell Reports. 36(4):109455. PubMed Wu J et al. 2018. Cell reports. 25(7):1966-1981 . PubMed Guo D, et al. 2022. Elife. 11:. PubMed
RRID: AB_2099568 (BioLegend Cat. No. 324707) AB_2099567 (BioLegend Cat. No. 324708)
Structure: Member of the epidermal growth factor receptor family. A receptor tyrosine kinase containing 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain. Predicted molecular weight approximately 148 kD
Distribution: Widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. Forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in b
Function: Induces extensive signaling cascade that can regulate growth and possibly differentiation
Ligand/Receptor: Heregulin
Modification: Extensively phosphorylated T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, Y1328
Biology Area: Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Carraway KL, et al. 1994. J. Biol. Chem. 269:14303. 2. Kraus MH, et al. 1989. Proc. Natl. Acad. Sci. USA 86:9193. 3. Chan SD, et al. 1995. J. Biol. Chem. 270:22608. 4. Tanner B, et al. 2006. J. Clin. Oncol. (Epub Aug. 8 2006)
Gene ID: 2065
UniProt: View information about erbB3/HER-3 on UniProt.org
Clone: 1B4C3
Regulatory Status: RUO
Other Names: erbB-3, erbB3, HER-3, HER3
Isotype: Mouse IgG2a, κ