Biolegend, 324801, Purified anti-human CD164 Antibody, 25μg

The 67D2 monoclonal antibody recognizes human CD164 also known as sialomucin CD164, MUC-24, and multi-glycosylated core protein 24. CD164 is a single pass transmembrane protein with short cytoplasmic tail that is highly N- and O-glycosylated. This protein contains sialic acid and a Ser-Gly motif that may serve as an attachment for a glycosaminoglycan side chain. Three splice variants of CD164 have been reported with apparent molecular weights ranging between 80-100 kD. CD164 is expressed in bone marrow, bone marrow stromal cells, and CD34+ hematopoietic cells myeloid and erythroid progenitors; and activated basophils. Expression has also been reported on a variety of carcinomas and leukemic cells and in the small intestine, colon, lung, and thyroid. CD164 plays a role in cell adhesion and proliferation and acts as a negative signaling molecule for hematopoietic progenitor cells. CD164 has also been reported to be involved in myogenic differentiation and cancer metastasis. The 67D2 antibody has been shown to be useful for the flow cytometric detection of human CD164, Western blotting under non-reducing conditions (detects an 80-100 kD protein as well as a high molecular weight aggregate of approximately 220 kD), and immunofluorescence.
25μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: T-47D breast carcinoma line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC - Verified WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3 under non-reducing conditions (detects an 80-100 kD protein as well as a high molecular weight aggregate of approximately 220 kD), and immunofluorescence3.
Application References(PubMed link indicates BioLegend citation): Watt SM, et al. 1998. Blood 92:849. Watt SM, et al. 2000. Blood 95:3113. Doyonnas R, et al. 2000. J. Immunol. 165:840. Vogel W, et al. 2002. Haematologica 88:126.
Product Citations: Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
RRID: AB_756020 (BioLegend Cat. No. 324802)
Structure: Single pass transmembrane protein with short cytoplasmic tail. Highly N- and O-glycosylated, contains sialic acid and a Ser-Gly motif that may serve as an attachment for a glycosaminoglycan side chain. Three splice variants have been reported with apparen
Distribution: Expressed in bone marrow, bone marrow stromal cells, and CD34+ hematopoietic cells myeloid and erythroid progenitors; activated basophils. Expressed on a variety of carcinomas and leukemic cells. Also expressed in the small intestine, colon, l
Function: Plays a role in cell adhesion and proliferation, negative signaling molecule for hematopoietic progenitor cells. Has also been reported to be involved in myogenic differentiation and cancer metastasis
Cell Type: Basophils, Hematopoietic stem and progenitors, Leukemia
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zannettino ACW, et al. 1998. Blood 92:2613. 2. Havens AM, et al. 2006. BMC Cancer 6:195. 3. Lee YN, et al. 2001. Mol. Cell. Biol. 21:7696.
Gene ID: 8763
UniProt: View information about CD164 on UniProt.org
Clone: 67D2
Regulatory Status: RUO
Other Names: Sialomucin CD164, MUC-24, multi-glycosylated core protein 24, MGC-24
Isotype: Mouse IgG1, κ