Biolegend, 325504, Biotin anti-human IgE Antibody, 100μg

IgE is the seventh immunoglobulin made by B cells in the immune response.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedELISA, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
Product Citations: Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed Harms RZ, et al. 2015. PLoS One. e0117335:10. PubMed Mencarelli A, et al. 2024. Nat Immunol. 693:25. PubMed Li T, et al. 2018. J Biol Chem. 293:1243. PubMed
RRID: AB_830848 (BioLegend Cat. No. 325503) AB_830849 (BioLegend Cat. No. 325504)
Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3497
UniProt: View information about IgE on UniProt.org
Clone: MHE-18
Regulatory Status: RUO
Other Names: Immunoglobulin E
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.