Biolegend, 325512, PerCP/Cyanine5.5 anti-human IgE Antibody, 100tests

IgE is the seventh immunoglobulin made by B cells in the immune response.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
Product Citations: Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
RRID: AB_11218987 (BioLegend Cat. No. 325511) AB_11219590 (BioLegend Cat. No. 325512)
Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3497
UniProt: View information about IgE on UniProt.org
Clone: MHE-18
Regulatory Status: RUO
Other Names: Immunoglobulin E
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.