Biolegend, 326307, PE anti-human CD252 (OX40L) Antibody, 25tests

OX40 ligand (OX40L) is a 35 kD type II transmembrane protein which belongs to TNF superfamily, also known as gp34, CD252, CD134L or TNFSF4. The OX40 ligand is expressed on activated B cells, activated endothelial cells, mature dendritic cells, activated CTLs, activated mast cells, and airway smooth muscle cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to regulate T cell proliferation and differentiation, and to enhance immunoglobulin secretion by activated B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Castellanos JG et al. 2018. Immunity. 49(6):1077-1089 . PubMed Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed Liang Y, et al. 2019. J Allergy Clin Immunol. 144:1025. PubMed Guo M, et al. 2021. Front Oncol. 11:632540. PubMed Valenzuela NM, et al. 2021. Front Immunol. 12:648946. PubMed Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
RRID: AB_2207272 (BioLegend Cat. No. 326307) AB_2207271 (BioLegend Cat. No. 326308)
Structure: NGF/TNF superfamily, 35 kD
Distribution: Activated B cells, activated endothelial cells, mature dendritic cells, airway smooth muscle cells
Function: B-cell/T-cell interaction, costimulation
Ligand/Receptor: OX40 (CD134)
Cell Type: B cells, Dendritic cells, Endothelial cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Imura A, et al. 1996. J. Exp. Med. 183:2185. 2. Ohshima Y, et al. 1997. J. Immunol. 159:3838. 3. Ito T, et al. 2005. J. Exp.Med. 202:1213. 4. Ito T, et al. 2006. Proc. Natl. Acad.Sci. USA. 103:13138.
Gene ID: 7292
UniProt: View information about CD252 on UniProt.org
Clone: 11C3.1
Regulatory Status: RUO
Other Names: Gp34, OX40L, CD134L, CD252, TNFSF4
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.