Biolegend, 327801, Purified anti-human CD104 Antibody, 25μg

CD104 is a 205 kD type I transmembrane glycoprotein, known as integrin β4 chain or β4 integrin, that associates with integrin α6 (CD49f) forms α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation and of cell proliferation and activation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
RRID: AB_893212 (BioLegend Cat. No. 327802)
Structure: Integrin β chain, 205 kD, forms heterodimer with integrin α6 (CD49f)
Distribution: Epithelial cells, endothelial cells, some tumor cells, pre-T cell subset
Function: Adhesion
Ligand/Receptor: Laminins, keratin filaments
Cell Type: Epithelial cells, Endothelial cells, T cells, Embryonic Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kippenberger S, et al. 2004. J. Invest. Dermatol. 123:444.2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
Gene ID: 3691
UniProt: View information about CD104 on UniProt.org
Clone: 58XB4
Regulatory Status: RUO
Other Names: Integrin β4, β4 integrin, TSP-1180, ITGB4
Isotype: Mouse IgG2a, κ