Biolegend, 328114, APC anti-human CD90 (Thy1) Antibody, 100tests

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34 + cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34 + CD90 + cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HEL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Application References(PubMed link indicates BioLegend citation): Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
Product Citations: Gómez-Aristizábal A, et al. 2016. PLoS One. 11: 0147868. PubMed Veraitch O, et al. 2017. Sci Rep. 7:42777. PubMed Melzer C, et al. 2020. Int J Mol Sci. :21. PubMed Crees ZD, et al. 2023. Nat Med. 29:869. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Huang J, et al. 2022. Clin Exp Pharmacol Physiol. 49:881. PubMed Li N, et al. 2023. Int J Mol Sci. 24: . PubMed Sluzalska K,et al. 2017. Sci Rep.. 10.1038/s41598-017-14004-9. PubMed Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed Jiang S, et al. 2021. Bioact Mater. 2711:6. PubMed Sluzalska KD, et al. 2017. Osteoarthritis Cartilage. 25:1890. PubMed Wei X, et al. 2010. Proc Natl Acad Sci U S A. 107:18874. PubMed Tanbakooei S, et al. 2022. J Reprod Infertil. 22:241. PubMed Chen GY, et al. 2022. Drug Des Devel Ther. 16:1743. PubMed Pustlauk W, et al. 2020. Sci Rep. 10:5951. PubMed de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed Sun Z, et al. 2022. Stem Cell Res Ther. 13:297. PubMed Xiao X, et al. 2019. Cell Discov. 5:2. PubMed Welsh JA, et al. 2022. Cell Rep Methods. 2:100136. PubMed Zhang X, et al. 2022. Nat Commun. 13:5211. PubMed Wu Y, et al. 2019. Nat Med. 25:776. PubMed Brandsma AM, et al. 2021. Cancer Discov. . PubMed Ragni E, et al. 2022. Biomolecules. 12:. PubMed Calandrini C, et al. 2020. Nat Commun. 11:1310. PubMed Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed Li J, et al. 2021. Mol Med Rep. 1:23. PubMed Zeng J, et al. 2020. Nat Med. 26:535. PubMed Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed Bian X, et al. 2021. Clin Transl Med. 11:e352. PubMed Ji S, et al. 2021. Clin Sci (Lond). 135:1127. PubMed Kink JA, et al. 2019. Biol Blood Marrow Transplant. 25:2124. PubMed Pfeiffenberger M, et al. 2020. ALTEX. 37:561. PubMed Haideri T, et al. 2022. Cell Rep Methods. 2:100290. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed Basile M, et al. 2020. J Cell Physiol. 235:4643. PubMed Boucher JM, et al. 2018. Cardiovasc Drugs Ther. 32:519. PubMed Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed Pezhouman A, et al. 2022. Cardiovasc Res. 118:828. PubMed Wilkinson AC, et al. 2019. Nature. 571:117. PubMed Wu SZ, et al. 2020. EMBO J. 39:e104063. PubMed Papait A, et al. 2022. Front Immunol. 13:960909. PubMed
RRID: AB_893440 (BioLegend Cat. No. 328113) AB_893431 (BioLegend Cat. No. 328114)
Structure: 25-35 kD glycoprotein, Ig superfamily
Distribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
Function: Regulate hematopoiesis and neural cell growth, cell adhesion
Ligand/Receptor: β3 integrin, β2 integrin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850.
Gene ID: 7070
UniProt: View information about CD90 on UniProt.org
Clone: 5E10
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ