Biolegend, 329801, Purified anti-human CD275 (B7-H2, B7-RP1, ICOSL) Antibody, 25μg

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human B7H2-mIg fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Qian X, et al. 2006. Eur J Immunol. 36:906-918. Ribot JC, et al. 2012. J. Immunol. 189:1202. PubMed.
Product Citations: Ribot J, et al. 2012. J Immunol. 189:1202. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
RRID: AB_960990 (BioLegend Cat. No. 329801)
Structure: Ig superfamily, B7 family, 40 kD
Distribution: B cells and dendritic cells
Function: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand/Receptor: ICOS
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Qian X, et al. 2006. Eur J Immunol. 36:906-918.
Gene ID: 23308
UniProt: View information about CD275 on UniProt.org
Clone: 9F.8A4
Regulatory Status: RUO
Other Names: B7-H2, B7H2, B7-RP1, ICOSL, ICOS-L
Isotype: Mouse IgG1, κ