Biolegend, 330101, Purified anti-human CD209 (DC-SIGN) Antibody, 25μg

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human DC-SIGN
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3.
Application References(PubMed link indicates BioLegend citation): Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Parker R, et al. 2020. bioRxiv. . PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Azari S, et al. 2021. MBio. 12:e0184921. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Chen W, Pilling D, and Gomer R 2017. BMC Immunol. . 10.1186/s12865-017-0230-z. PubMed Song J, et al. 2022. PLoS Biol. 20:e3001805. PubMed
RRID: AB_1134253 (BioLegend Cat. No. 330102)
Distribution: Dendritic cells
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.
Gene ID: 30835
UniProt: View information about CD209 on UniProt.org
Clone: 9E9A8
Regulatory Status: RUO
Other Names: Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
Isotype: Mouse IgG2a, κ