Biolegend, 330505, PE anti-human TCL1 Antibody, 25tests

TCL1, also known as T-cell leukemia/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kD beta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia (T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11;q32.1) or t(14;14)(q11;q32.1), TCL1 is normally expressed in immature cortical thymocytes, activated peripheral T-cells,  pro-B cells, and naive mantle zone B cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt and TCL1 interact via Akt’s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasma membrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase (PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blocking PKCθ. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma, chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: outer α-loop region of TCL1 peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.
Application References(PubMed link indicates BioLegend citation): Herling M, et al. 2008. Blood 111:328 Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123
Product Citations: O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
RRID: AB_2204407 (BioLegend Cat. No. 330506)
Structure: Beta barrel protein, 14kD
Distribution: Cytoplasm
Function: Enhances Akt mediated cell survival, stabilizes mitochondrial membrane potential
Interaction: Akt1, Akt2, Akt3, caco2
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Narducci MG et al. 2000. Cancer Research 60:2095. 2. Teitell MA et al. 2005 Nat. Rev. Cancer 5:640 3. Laine J et al.2000. Molecular Cell 6:395 4. Virgilio L et al. 1994. Proc. Natl. Acad. Sci. 91:12530 5. Masayuk N et al. 2007 FASEB J. 21:2273 6. Despouy G et al. 2007 Blood 110:4406 7. Rhine S et al. 2006 Blood 108:1991
Gene ID: 8115
UniProt: View information about TCL1 on UniProt.org
Clone: 1-21
Regulatory Status: RUO
Other Names: T-cell leukemia/lymphoma protein 1A, Protein p14 TCL1, Oncogene TCL1, TCL-1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.