Biolegend, 331101, Purified anti-human/mouse CXCR7 Antibody, 25μg

CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR). CXCR7 binds with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors. CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and other cell types.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR7 transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Soehnlein O, et al. 2013. EMBO Mol. Med. 5:471. (FC) PubMed Balabanian K, et al. 2012. J. Transl. Med. 10:251. (FC) PubMed
Product Citations: Zabel B, et al. 2009. J Immunol. 183:3204. PubMed Berahovich R, et al. 2010. J Immunol. 185:5130. PubMed
RRID: AB_2089777 (BioLegend Cat. No. 331102)
Structure: G protein-coupled receptor super family
Distribution: Monocytes, B cells, T cells and mature dendritic cells
Ligand/Receptor: CXCL12/SDF-1 and CXCL11/I-TAC
Cell Sources: On a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells
Cell Type: B cells, Dendritic cells, Monocytes, T cells
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Burns JM, et al. 2006. J. Exp. Med. 203:2201. 2. Infantino S, et al. 2006. J. Immunol. 176:2197.
Regulation: Regulates the trafficking and activation of leukocytes
Gene ID: 1277857007
UniProt: View information about CXCR7 on UniProt.org
Clone: 8F11-M16
Regulatory Status: RUO
Other Names: Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159
Isotype: Mouse IgG2b, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.