Biolegend, 331802, Purified anti-human CD319 (CRACC) Antibody, 100μg

CD319 is a single-pass type l transmembrane glycoprotein, expressed on NK cells, subsets of mature dendritic cells, activated B cells, and cytotoxic lymphocytes, but not in promyelocytic, B or T cell lines. Expression is highest in the spleen, lymph nodes, and peripheral blood leukocytes, and lowest in bone marrow. Additionally, it is expressed in the small intestine, stomach, appendix, lung, and trachea. CD319 is tyrosine phosphorylated in activated NK cells and is associated with 19 and 39 kD proteins. CD319 has homology with the CD2 family of receptors within the Ig superfamily. Some of the CD2 members stimulate cytotoxicity through the CD319 associated protein.
100μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CRACC-Human IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Bouchon A, et al. 2001. J. Immunol. 167:551721. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002. Veillette A. 2006. Immunol. Rev. 214:2234.
Product Citations: Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed Quentmeier H, et al. 2019. Sci Rep. 9:8218. PubMed Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
RRID: AB_961330 (BioLegend Cat. No. 331802)
Structure: Ig superfamily, 66 kD.
Distribution: NK cells, subsets of mature dendritic cells, activated B cells, cytotoxic lymphocytes but not in promyelocytic, B or T cell lines.
Function: Regulates T cell and NK cell functions.
Cell Type: B cells, Dendritic cells, Lymphocytes, NK cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Bouchon A, et al. 2001. J. Immunol. 167:551721. 2. Tassi I and Colonna M. 2005. J. Immunol. 175:79968002. 3. Veillette A. 2006. Immunol. Rev. 214:2234.
Gene ID: 57823
UniProt: View information about CD319 on UniProt.org
Clone: 162.1
Regulatory Status: RUO
Other Names: CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24, SLAMF7
Isotype: Mouse IgG2b, κ