Biolegend, 331907, PE anti-human CD335 (NKp46) Antibody, 25tests

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3 - CD56 + NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NKp46-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 9E2 has been shown to block NK activation through NKp46.6
Application References(PubMed link indicates BioLegend citation): Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. Kalberer CP, et al. 2003. Blood 102:127. Chen Y, et al. 2007. J. Immunol. 179:2766. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Achdout H. et al. 2010. J. Virol. 84:3993.
Product Citations: Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed Lee Y, et al. 2020. J Innate Immun. 1:. PubMed Mayassi T, et al. 2019. Cell. 176:967. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Spallanzani R, et al. 2015. J Immunol. 195: 2141-2148. PubMed Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed Rajashekar JK, et al. 2021. Cell Host Microbe. 29(6):904-916.e6. PubMed Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed Sadallah S, et al. 2016. J Immunol. 197: 1663 - 1671. PubMed Zanetti S, et al. 2016. J Biol Chem. 291: 16541 - 16552. PubMed
RRID: AB_1027679 (BioLegend Cat. No. 331907) AB_1027666 (BioLegend Cat. No. 331908)
Structure: Type I membrane glycoprotein (46 kD)
Distribution: Expressed on resting and activated NK cells
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147. 2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 3. Sivori S. 1999. Eur. J. Immunol. 29:1656.
Gene ID: 9437
UniProt: View information about CD335 on UniProt.org
Clone: 9E2
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.