Biolegend, 332802, Purified anti-human IRF1 Antibody, 100μg

IRF1 is the first identified member of the interferon regulatory transcription factor family. Its expression is induced in response to pathogen infection or the stimulation of several cytokines, including type I and II IFN, IL-1, IL-6, and TNF-α. Upon stimulation, IRF1 is transcriptionally activated by STAT1 and NFκB transcription factors via the GAS (interferon-gamma-activated sequence) element in the IRF1 promoter. IRF1 plays important roles in mediating immune response during infection and development of T cells and NK cells. In addition to the immunomodulatory functions, IRF1 has also shown to be involved in the regulation of cell cycle progression, tumor suppression, and apoptosis. IRF1-mediated transcription can be repressed by IRF2 through competing for the same binding region on the promoters.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human IRF1 recombinant protein (147-325 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality Tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2904353 (BioLegend Cat. No. 332802)
Structure: 325 amino acids, predicted MW is 37 kD and observed MW is 45 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.
Distribution: Nucleus
Function: IRF1 is a transcription factor, regulating expression of IFN and IFN-inducible genes. It is also involved in regulating cell proliferation, apoptosis, tumor suppression, and various immune responses.
Interaction: Forms homodimers. Interacts with p300, MyD88, and PIAS3.
Cell Type: Leukocytes, Macrophages, Monocytes, NK cells, T cells, Th1, Tregs
Biology Area: Adaptive Immunity, Immunology, Innate Immunity, Transcription Factors
Antigen References: 1. Yarilina A, et al. 2008. Nat. Immunol. 9:378. 2. Kano S, et al. 2008. Nat. Immunol. 9:34.
Gene ID: 3659
UniProt: View information about IRF1 on UniProt.org
Clone: W17068A
Regulatory Status: RUO
Other Names: Interferon regulatory factor 1 (IRF-1)
Isotype: Rat IgG2a, κ