Biolegend, 334006, PE anti-human CD167a (DDR1) Antibody, 100tests

The 51D6 monclonal antibody recognizes human CD167a also known as discoidin domain receptor, DDR1, neuroepithelial tyrosine kinase, epithelial-specific receptor kinase, tyrosine kinase receptor E (trkE), cell adhesion kinase (CAK), PTK3, and RTK6. CD167a is a membrane type II receptor kinase, containing a factor VIII-like domain. At least five isoforms are generated through alternative splicing of the kinase domain of human DDR1 gene. These isoforms are named wtih suffixes a to e. CD167a is expressed on epithelial cells, keratinocytes, leukocytes, monocytes and has been reported to be overexpressed in some breast carcinomas. CD167a expression can be upregulated by p53. CD167a has been reported to interact with a variety of proteins including collagen type II alpha 1, collagen type III alpha 1, collagen type V alpha 2, collagen type XI alpha 1, phospholipase gamma 1, SHC, and the lipid-anchored docking protein FRS2. CD167a can be phosphorylated on Y513. Animal studies suggest that CD167a is involved in arterial wound healing. The 51D6 antibody has been shown to be useful for flow cytometric detection of human CD167a as well as immunoprecipitation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH-3T3 cells transfected with human DDR1 (CD167a)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) applications include:immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Rudra-Ganguly N, et al. 2014. PLoS One. 9:111515. PubMed
Product Citations: Rudra-Ganguly N, et al. 2014. PLoS One. 9:111515. PubMed
RRID: AB_2261656 (BioLegend Cat. No. 334005) AB_2092093 (BioLegend Cat. No. 334006)
Structure: Membrane Type II receptor kinase, containing a factor VIII-like domain. Three isoforms have been reported. Predicted molecular weights are: isoform 1, approximately 101.5 kD, isoform 2, approximately 101 kD, and isoform 3 approximately 97 kD.
Distribution: Epithelial cells, keratinocytes, and activated leukocytes. Overexpressed in some breast carcinomas. Expression can be upregulated by p53.
Function: Receptor membrane tyrosine kinase. May be involved in arterial wound repair.
Interaction: CD167a has been reported to interact with a variety of proteins including collagen type II alpha 1, collagen type III alpha 1, collagen type V alpha 2, collagen type XI alpha 1, phospholipase gamma 1, SHC, lipid-anchored docking protein FRS2
Modification: Phosphorylation (Y513)
Cell Type: Epithelial cells, Leukocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. DiMarco E, et al. 1993. J. Biol. Chem. 268:24290. 2. Hou G, et al. 2001. J. Clin. Invest. 107:727. 3. Johnson JD, et al. 1993. Proc. Natl. Acad. Sci. USA 90:5677. 4. Sakuma S, et al. 1996. FEBS Lett. 398:165.
Gene ID: 780
UniProt: View information about CD167a on UniProt.org
Clone: 51D6
Regulatory Status: RUO
Other Names: Discoidin domain receptor, DDR1, neuroepithelial tyrosine kinase, epithelial-specific receptor kinase, tyrosine kinase receptor E (trkE), cell adhesion kinase (CAK), PTK3, RTK6
Isotype: Mouse IgM, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.