Biolegend, 334107, APC anti-human CD71 Antibody, 25tests

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NALM-6 pre-B cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations: Scanlon VM, et al. 2022. Sci Rep. 12:16218. PubMed Wang S, et al. 2023. Sci Adv. 9:eade2540. PubMed Jiang J, et al. 2023. iScience. 26:106425. PubMed Chernyshev M, et al. 2023. Nat Commun. 14:2249. PubMed Hamilton MC, et al. 2023. Cell Genom. 3:100304. PubMed Thiramanas R, et al. 2020. Cells. 9: . PubMed Molugu K, et al. 2020. Biophys J. 2086:118. PubMed Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed Molugu K, et al. 2022. GEN Biotechnol. 1:176. PubMed Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed Coelho PP, et al. 2022. Nat Commun. 13:3812. PubMed Grajcarek J, et al. 2019. Nat Commun. 3.788888889. PubMed Kwon E, et al. 2018. Nat Commun. 9:1734. PubMed Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed Matsuo S, et al. 2021. PLoS One. e0247595:16. PubMed Woods E, et al. 2021. NPJ Vaccines. 6:117. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Tian P, et al. 2022. Front Cell Dev Biol. 10:835321. PubMed
RRID: AB_10916388 (BioLegend Cat. No. 334107) AB_10915138 (BioLegend Cat. No. 334108)
Structure: Transferrin receptor family, type II glycoprotein, 95 kD
Distribution: Proliferating cells, reticulocytes, erythroid precursors
Function: Mediates uptake of iron
Ligand/Receptor: Transferrin
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175. 2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.
Gene ID: 7037
UniProt: View information about CD71 on UniProt.org
Clone: CY1G4
Regulatory Status: RUO
Workshop: A015
Other Names: T9, Transferrin receptor
Isotype: Mouse IgG2a, κ