Biolegend, 334204, Biotin anti-human CD202b (Tie2/Tek) Antibody, 100μg

CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant extracellular domain of human Tie2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA
Application References(PubMed link indicates BioLegend citation): Peters KG, et al. 1998. Br. J. Cancer. 77:51. Wong AL, et al. 1997. Circ. Res. 81:567. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.
Product Citations: Hoover MY, et al. 2023. Nat Protoc. . PubMed Tevlin R, et al. 2023. Sci Rep. 13:2097. PubMed Goodnough LH, et al. 2020. JBMR Plus. 4:e10398. PubMed Ambrosi TH, et al. 2020. Aging Cell. 19:e13164. PubMed
RRID: AB_1186046 (BioLegend Cat. No. 334204)
Structure: 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution: Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
Function: Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand/Receptor: Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1.De Palma M, et al. 2005. Cancer Cell. 8:211 2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168 3. Hsu HC, et al. 2000. Blood. 96:3757 4. Arai F, et al. 2004. Cell. 118:149
Gene ID: 7010
UniProt: View information about CD202b on UniProt.org
Clone: 33.1 (Ab33)
Regulatory Status: RUO
Other Names: Tie2, TEK
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.