Biolegend, 334601, Purified anti-human FcεRIα Antibody, 25μg

High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.
25μg
Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4
Application References(PubMed link indicates BioLegend citation): Yamaguchi M, et al. 1999. J. Immunol. 162:5455. Suzukawa M, et al. 2005. Int. Immunol. 17:1249. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
Product Citations: Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
RRID: AB_1227649 (BioLegend Cat. No. 334602)
Structure: Ig superfamily, 50 kD
Distribution: Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients
Function: Bind IgE, trigger IgE-mediated allergic response
Ligand/Receptor: IgE
Cell Type: Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
Biology Area: Immunology
Molecular Family: Fc Receptors
Antigen References: 1. Riske F, et al. 1991. J. Biol. Chem. 266:11245 2. Gounni AS, et al. 2001. FASEB J. 15:940. 3. Maurer D, et al. 1996. J. Immunol. 157:607 4. Maurer d, et al. 1994. J. Exp. Med. 179:745 5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.
Gene ID: 2205
UniProt: View information about FcepsilonRIalpha on UniProt.org
Clone: AER-37 (CRA-1)
Regulatory Status: RUO
Other Names: FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
Isotype: Mouse IgG2b, κ