Biolegend, 335853, PE anti-human IDO1 Antibody, 25tests

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO1 is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. It is one of the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein, full length human IDO
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3662230 (BioLegend Cat. No. 335853) AB_3662230 (BioLegend Cat. No. 335854)
Structure: Heme-containing enzyme of 403 amino acids with a predicted molecular weight of 45 kD.
Distribution: Cytoplasm
Function: Dioxygenase, Oxidoreductase
Ligand/Receptor: Heme, Iron, Metal binding
Cell Type: Dendritic cells, Eosinophils, Langerhans cells, Macrophages, Schwann Cells, T cells
Biology Area: Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family: Enzymes and Regulators
Antigen References: Yamazaki F, et al.1985. The Biochemical Journal. 230: 635–8. Prendergast GC, et al. 2014. Frontiers in Immunology. 5:585. Habara-Ohkubo A, et al. 1991. Gene. 105:221. Munn DH, et al. 2002. Science. 297:1867. Frumento G, et al. 2002. J Exp Med. 196:459. Muller AJ, et al. 2005. Nature Med. 11:312. Löb S, et al. 2009. Nature Rev Cancer. 9:445-52.
Gene ID: 15930
UniProt: View information about IDO1 on UniProt.org
Clone: W20099D
Regulatory Status: RUO
Other Names: Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype: Rat IgG2b, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.