Biolegend, 337202, Purified anti-human CD11c Antibody, 100μg

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. CD11c non-covalently associates with integrin β 2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.
Application References(PubMed link indicates BioLegend citation): Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. Rezzonico R, et al. 2001. Blood 97:2932. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed Gruber CN, et al. 2020. Cell. 183:982. PubMed Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed Henrick BM, et al. 2021. Cell. . PubMed Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID: AB_1236381 (BioLegend Cat. No. 337202)
Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function: Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.
Gene ID: 3687
UniProt: View information about CD11c on UniProt.org
Clone: Bu15
Regulatory Status: RUO
Workshop: V S143
Other Names: Integrin αx subunit, ITGAX, CR4, p150
Isotype: Mouse IgG1, κ