Biolegend, 338312, APC anti-human CD226 (DNAM-1) Antibody, 100tests

DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications include: block NK cell-mediated lysis.
Application References(PubMed link indicates BioLegend citation): Fuchs A, et al. 2004. J. Immunol. 172:3994.
Product Citations: Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed Sutavani R, et al. 2013. J Immunol. 191:5895. PubMed Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed Choi JW, et al. 2019. BMC Cancer. 19:817. PubMed Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Alonso R, et al. 2022. Blood Adv. :. PubMed Elias S, et al. 2020. Haematologica. . PubMed
RRID: AB_2561951 (BioLegend Cat. No. 338311) AB_2561952 (BioLegend Cat. No. 338312)
Structure: Ig domain containing adhesion molecule
Distribution: Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells
Function: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
Interaction: Interacts with LFA-1.
Ligand/Receptor: PVR (CD155) and Nectin-2 (CD112)
Cell Type: B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Shibuya A et al. 1996. Immunity 4:573. 2. Tahara-Hanaoka S et al. 2004. Int. Immunol. 16:533. 3. Pende D et al. 2005. Mol. Immunol. 42:463.
Gene ID: 10666
UniProt: View information about CD226 on UniProt.org
Clone: 11A8
Regulatory Status: RUO
Other Names: DNAM-1, PTA1, TLiSA1
Isotype: Mouse IgG1, κ