Biolegend, 338326, Biotin anti-human CD226 (DNAM-1) Antibody, 100μg

DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: block NK cell-mediated lysis.
Application References(PubMed link indicates BioLegend citation): Fuchs A, et al. 2004. J. Immunol. 172:3994.
RRID: AB_2721495 (BioLegend Cat. No. 338326)
Structure: Ig domain containing adhesion molecule
Distribution: Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells
Function: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
Interaction: Interacts with LFA-1.
Ligand/Receptor: PVR (CD155) and Nectin-2 (CD112)
Cell Type: B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Shibuya A et al. 1996. Immunity 4:573. 2. Tahara-Hanaoka S et al. 2004. Int. Immunol. 16:533. 3. Pende D et al. 2005. Mol. Immunol. 42:463.
Gene ID: 10666
UniProt: View information about CD226 on UniProt.org
Clone: 11A8
Regulatory Status: RUO
Other Names: DNAM-1, PTA1, TLiSA1
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.