Biolegend, 339203, PE anti-human CD328 (Siglec-7) Antibody, 25tests

Siglec-7, also known as p75/AIRM1, is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs). It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Hartana CA, et al. 2022. Cell Rep. 40:111126. PubMed Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed Rosenstock P, et al. 2017. Immunol Res. 65:1017. PubMed
RRID: AB_1501164 (BioLegend Cat. No. 339203) AB_1501160 (BioLegend Cat. No. 339204)
Structure: Type I transmembrane protein, Siglec family member, 75 kD
Distribution: NK cells, monocytes
Function: Inhibitory receptor of NK cells
Ligand/Receptor: Sialylated glycans (with α2,8 disialyl and α2,6 sialyl residues)
Cell Type: Monocytes, NK cells
Biology Area: Immunology
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: Avril T, et al. 2006. Infection and Immunity 74:4133 Avril T, et al. 2004. J. Immunol. 173:6841 Yamaji T, et al. 2005. Glycobiology 15:667
Gene ID: 27036
UniProt: View information about CD328 on UniProt.org
Clone: 6-434
Regulatory Status: RUO
Workshop: VIII 80652
Other Names: Siglec7 (Sialic-binding Ig like lectin 7), p75, AIRM1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.