Biolegend, 339216, PerCP/Cyanine5.5 anti-human CD328 (Siglec-7) Antibody, 100tests

Siglec-7, also known as p75/AIRM1, is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs). It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2814250 (BioLegend Cat. No. 339215) AB_2814251 (BioLegend Cat. No. 339216)
Structure: Type I transmembrane protein, Siglec family member, 75 kD
Distribution: NK cells, monocytes
Function: Inhibitory receptor of NK cells
Ligand/Receptor: Sialylated glycans (with α2,8 disialyl and α2,6 sialyl residues)
Cell Type: Monocytes, NK cells
Biology Area: Immunology
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: Avril T, et al. 2006. Infection and Immunity 74:4133 Avril T, et al. 2004. J. Immunol. 173:6841 Yamaji T, et al. 2005. Glycobiology 15:667
Gene ID: 27036
UniProt: View information about CD328 on UniProt.org
Clone: 6-434
Regulatory Status: RUO
Workshop: VIII 80652
Other Names: Siglec7 (Sialic-binding Ig like lectin 7), p75, AIRM1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.