Biolegend, 339802, Purified anti-BrdU Antibody, 100μg

BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.
Application References(PubMed link indicates BioLegend citation): Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517. Ejassi-Lassallette A, et al. 2011. 22:245. PubMed Ma L, et al. 2012 PNAS. 109:2090. PubMed.
Product Citations: Fu S, et al. 2023. Cells. 12:. PubMed Avalos A, et al. 2022. JCI Insight. 7: . PubMed Yu X, et al. 2023. Br J Pharmacol. 180:161. PubMed AR F, et al. 2016. Sci Rep. 6: 32900. PubMed Ejlassi-Lassallette A, et al. 2011. Mol Biol Cell. 1.086805556. PubMed Ma L, et al. 2012. Proc Natl Acad Sci U S A. 109:2090. PubMed Yoon J, et al. 2022. Cell Death Differ. . PubMed
RRID: AB_1595600 (BioLegend Cat. No. 339801) AB_1595495 (BioLegend Cat. No. 339802)
Structure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Function: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.
Gene ID: NA
Clone: Bu20a
Regulatory Status: RUO
Other Names: Bromodeoxyuridine
Isotype: Mouse IgG1, κ