Biolegend, 340304, PE anti-human CD307e (FcRL5) Antibody, 100tests

FcRL5, also known as CD307e, CD307, IRTA2, FcRH5, and BXMAS1, is a 106 kD type I transmembrane glycoprotein composed of six-eight extracellular Ig like C2 domains and two cytoplasmic ITIM motifs. The FcRL5 gene is located on chromosome 1q21 and is normally expressed by tonsillar plasma cells and germinal center B cells. It has been suggested that FcRL5 contributes to B cell maturation and B cell receptor modulation. The identification of FcRL5 on a number of transformed cell types including hairy cell leukemia, mantle cell lymphoma, chronic lymphocytic lymphoma, and Burkitt’s lymphoma has prompted interest in it as a potential biomarker or target for immunotherapy. FcRL5 binds all aggregated IgG isotypes.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: P815 cells transfected with FcRL5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation and blocking1. mAb 509f6 is able to block the binding of FcRL5 to all aggregated IgG isotypes.1
Application References(PubMed link indicates BioLegend citation): Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)
Product Citations: Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
RRID: AB_2104588 (BioLegend Cat. No. 340304)
Structure: 106 kD, Fc receptor like, type 1 transmembrane protein, 6-8 Ig like C2 domains, 2 cytoplasmic ITIM motifs.
Distribution: Germinal center B cells, tonsillar plasma cells.
Ligand/Receptor: Aggregated IgG
Cell Type: B cells, Plasma cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Davis R, et al. 2007. Annu. Rev. Immunol. 207:528. 2. Mohan J, et al. 2006. Blood 107:4433. 3. Ise T, et al. 2007. Leukemia. 21:169.
Gene ID: 83416
UniProt: View information about CD307e on UniProt.org
Clone: 509f6
Regulatory Status: RUO
Other Names: CD307, IRTA2, FcRH5, IFGP5, BXMAS1
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.