Biolegend, 340306, APC anti-human CD307e (FcRL5) Antibody, 100tests

FcRL5, also known as CD307e, CD307, IRTA2, FcRH5, and BXMAS1, is a 106 kD type I transmembrane glycoprotein composed of six-eight extracellular Ig like C2 domains and two cytoplasmic ITIM motifs. The FcRL5 gene is located on chromosome 1q21 and is normally expressed by tonsillar plasma cells and germinal center B cells. It has been suggested that FcRL5 contributes to B cell maturation and B cell receptor modulation. The identification of FcRL5 on a number of transformed cell types including hairy cell leukemia, mantle cell lymphoma, chronic lymphocytic lymphoma, and Burkitt’s lymphoma has prompted interest in it as a potential biomarker or target for immunotherapy. FcRL5 binds all aggregated IgG isotypes.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: P815 cells transfected with FcRL5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation and blocking1. mAb 509f6 is able to block the binding of FcRL5 to all aggregated IgG isotypes.1
Application References(PubMed link indicates BioLegend citation): Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)
Product Citations: Hata K, et al. 2023. Front Immunol. 14:1145814. PubMed Jiang D, et al. 2023. Nat Commun. 14:3642. PubMed SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
RRID: AB_2564326 (BioLegend Cat. No. 340305) AB_2564327 (BioLegend Cat. No. 340306)
Structure: 106 kD, Fc receptor like, type 1 transmembrane protein, 6-8 Ig like C2 domains, 2 cytoplasmic ITIM motifs.
Distribution: Germinal center B cells, tonsillar plasma cells.
Ligand/Receptor: Aggregated IgG
Cell Type: B cells, Plasma cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Davis R, et al. 2007. Annu. Rev. Immunol. 207:528. 2. Mohan J, et al. 2006. Blood 107:4433. 3. Ise T, et al. 2007. Leukemia. 21:169.
Gene ID: 83416
UniProt: View information about CD307e on UniProt.org
Clone: 509f6
Regulatory Status: RUO
Other Names: CD307, IRTA2, FcRH5, IFGP5, BXMAS1
Isotype: Mouse IgG2a, κ