Biolegend, 342301, Purified anti-human CD66a/c/e Antibody, 25μg

The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Shey MS, et al. 2012. J Immunol Methods. 376:79. PubMed.
Product Citations: Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed Obiedat A, et al. 2020. J Mol Med (Berl). 98:135. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Shey M, et al. 2012. J Immunol Methods. 376:79. PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
RRID: AB_1626265 (BioLegend Cat. No. 342302)
Structure: Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution: Granulocytes, epithelial cells
Function: Homophilic and heterophilic adhesion
Ligand/Receptor: CD66a-e, CD62E, galectins
Cell Type: Epithelial cells, Granulocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
Gene ID: 634
UniProt: View information about CD66a CD66c CD66e on UniProt.org
Clone: ASL-32
Regulatory Status: RUO
Other Names: CEACAM1/5/6, BGP, NCA
Isotype: Mouse IgG2b, κ