Biolegend, 342609, PerCP/Cyanine5.5 anti-human HLA-E Antibody, 25tests

HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199. Wooden SL, et al. 2005. J. Immunol. 175:1383. Monaco EL, et al. 2008. J. Immunol. 181:5442. Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed
Product Citations: Rahim MMA, et al. 2020. J Virol. 94:00:00. PubMed
RRID: AB_2565434 (BioLegend Cat. No. 342609) AB_2565435 (BioLegend Cat. No. 342610)
Structure: HLA family, Ig superfamily, 42 kD
Distribution: Broadly expressed
Function: Mediate cell recognition by NK cells
Ligand/Receptor: CD94/NKG2
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415. 2. Koller BH, et al. 1988. J. Immunol. 141:897.
Gene ID: 3133
UniProt: View information about HLA-E on UniProt.org
Clone: 3D12
Regulatory Status: RUO
Other Names: HLA class I histocompatibility antigen, alpha chain E, HLAE
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.