Biolegend, 343002, Purified anti-human CD209 (DC-SIGN) Antibody, 100μg

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.
100μg
Verified Reactivity: Human
Reported Reactivity: Pigtailed Macaque
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human DC-SIGN
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Obradovic MMS, et al. 2019. Nature. 540:567. PubMed
RRID: AB_2074330 (BioLegend Cat. No. 343002)
Distribution: Dendritic cells
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.
Gene ID: 30835
UniProt: View information about CD209 on UniProt.org
Clone: DCS-8C1
Regulatory Status: RUO
Other Names: Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
Isotype: Mouse IgG1, κ