Biolegend, 343101, Purified anti-human CD7 Antibody, 25μg

CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KG1a cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested PG - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported (for the relevant formats) applications include proteogenomics2.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University Press. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed Sigal N, et al. 2022. Methods Mol Biol. 2543:113. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Baran N, et al. 2022. Nat Commun. 13:2801. PubMed Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed Henrick BM, et al. 2021. Cell. . PubMed Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
RRID: AB_1659214 (BioLegend Cat. No. 343102)
Structure: Ig superfamily, type I transmembrane glycoprotein, 40 kD
Distribution: T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets
Function: T cell activation
Ligand/Receptor: Cytoplasmic domain associates with P13-kinase
Cell Type: Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. 2. Stillwell R, et al. 2001. Immunol. Res. 24:31. 3. Rabinowich H, et al. 1994. J. Immunol. 152:517.
Gene ID: 924
UniProt: View information about CD7 on UniProt.org
Clone: CD7-6B7
Regulatory Status: RUO
Workshop: IV T-164
Other Names: gp40
Isotype: Mouse IgG2a, κ