Biolegend, 343301, Purified anti-human HLA-A2 Antibody, 25μg

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells, playing an important role in cell-mediated immune responses and tumor surveillance.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Papain solubilized HLA-A2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles. Additional reported applications (for the relevant formats) include: immunoprecipitation3.
Application References(PubMed link indicates BioLegend citation): Brodsky FM, et al. 1979. Immunol. Rev. 47:3. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations: Chou JM, et al. 2022. Front Microbiol. 13:883597. PubMed Willimsky G, et al. 2021. Elife. 10:. PubMed Jensen SM, et al. 2018. Front Immunol. 9:2697. PubMed Reithofer M, et al. 2020. Eur J Immunol. 51:721. PubMed Chheda ZS, et al. 2018. J Exp Med. 9:19490. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Matsuoka K, et al. 2022. J Biol Chem. :101724. PubMed
RRID: AB_1659243 (BioLegend Cat. No. 343302)
Structure: Ig superfamily
Distribution: A*02 individuals leukocytes
Function: Antigen presentation
Ligand/Receptor: CD8, CD3/TCR
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3105
UniProt: View information about HLA-A2 on UniProt.org
Clone: BB7.2
Regulatory Status: RUO
Other Names: MHC-class I
Isotype: Mouse IgG2b, κ