Biolegend, 343306, PE anti-human HLA-A2 Antibody, 100tests

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells, playing an important role in cell-mediated immune responses and tumor surveillance.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Papain solubilized HLA-A2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles. Additional reported applications (for the relevant formats) include: immunoprecipitation3.
Application References(PubMed link indicates BioLegend citation): Brodsky FM, et al. 1979. Immunol. Rev. 47:3. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations: Sun Y, et al. 2023. Sci Adv. 9:eade7151. PubMed Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed Xiao C, et al. 2023. Nat Aging. 3:418. PubMed Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed Ameres S, et al. 2014. J Immunol. 192:5894. PubMed Schuler P, et al. 2014. Clin Cancer Res. 20:2433. PubMed Gu C, et al. 2019. J Immunol. 203:389. PubMed Wang X, et al. 2011. Blood. 117:1888. PubMed Xiao C, et al. 2022. iScience. 25:103934. PubMed Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed Klatt MG, et al. 2020. J Proteomics. 228:103921. PubMed Abdelaziz MO, et al. 2019. Front Immunol. 1.65. PubMed Lai J, et al. 2016. Blood. 128: 1396 - 1407. PubMed Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed Pettmann J, et al. 2021. eLife. 10:00. PubMed
RRID: AB_1877228 (BioLegend Cat. No. 343305) AB_1877227 (BioLegend Cat. No. 343306)
Structure: Ig superfamily
Distribution: A*02 individuals leukocytes
Function: Antigen presentation
Ligand/Receptor: CD8, CD3/TCR
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3105
UniProt: View information about HLA-A2 on UniProt.org
Clone: BB7.2
Regulatory Status: RUO
Other Names: MHC-class I
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.