Biolegend, 344002, Purified anti-human CD73 (Ecto-5'-nucleotidase) Antibody, 100μg

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Verified Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include:immunofluorescence3. Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.
Application References(PubMed link indicates BioLegend citation): Nakamura T, et al. 1993. J. Immunol. 151:6933. Liao J, et al. 2011. J Endod. 37:1217. PubMed Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations: Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Liao J, et al. 2011. J Endod. 37:1217. PubMed Heesters BA, et al. 2021. J Exp Med. 218:. PubMed Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed Garita-Hernández M, et al. 2021. Front Cell Neurosci. 15:648210. PubMed Xue G, et al. 2021. Nat Biomed Eng. 5:1306. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Messaoudi N, et al. 2020. Oncoimmunology. 9:1746138. PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID: AB_2154067 (BioLegend Cat. No. 344002)
Structure: GPI-linked 5'-nucleotidase, 70 kD
Distribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication. 2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755. 3. Gutensohn W, et al. 1995. Cell Immunol. 161:213. 4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
Gene ID: 4907
UniProt: View information about CD73 on UniProt.org
Clone: AD2
Regulatory Status: RUO
Workshop: V B-CD73.3
Other Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype: Mouse IgG1, κ